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| Anesthetic post-conditioning at the initiation of CPR attenuates cerebral injuries in a rat model of cardiac arrest |
| Li Heng-jie, Mao Hui, Cai Wen-wei, Yang Yan, Wei Hong-yan, Li Bo, Lu Yuan-zheng, Liao Xiao-xing |
| Department of Emergency, Zhejiang Province People′s Hospital, Hangzhou 310014, China |
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Abstract Objective In this study, we hypothesized that anesthetic post-conditioning with sevoflurane at the initiation of cardio-pulmonary resuscitation (CPR) will attenuate cerebral injuries in the model of cardiac arrest. Methods The cardiac arrest model was established through asphyxia. Forty male Wistar rats were assigned to two groups randomly. Rats in the sevoflurane (anesthetic post-conditioning, APoC) group inhaled 1 MAC of sevoflurane during CPR. Rats in the control (CON) group did not receive inhaled sevoflurane during CPR. After return of spontaneous circulation (ROSC), the mean arterial pressure (MAP) and heart rate (HR) was recorded. Before cardiac arrest (baseline) as well as ROSC 1 h and ROSC 4 h, cardiac function was measured. Neurologic deficit scores (NDS) was assessed at 24 and 72 hours after ROSC. At 72 hours after ROSC, coronal brain sections were analyzed by counting TUNEL positive (i.e, apoptotic) cells and Nissl positive (i.e, viable) cells. Results There was no statistical difference in MAP and HR between the two groups. Rats in the APoC group had a thinner LVPW (P<0.05) and a higher EDV (P<0.01) compared to the CON group. The apoptotic cells were less and the viable neurons were more in the APoC group compared with CON group (P<0.05). The NDS was higher in the APoC group at ROSC 24 h and ROSC 72 h (P<0.05). Conclusion Volatile anesthetic sevoflurane, when administered at initiation of CPR, improves NDS associated with reducing neuronal damage.
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Corresponding Authors:
Liao Xiao-xing, E-mail: liaowens@163.com
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