头部浅低温联合胰岛素样生长因子-1对大鼠全脑缺血再灌注损伤的影响

doi:10.3969/j.issn.1002-1949.2018.03.016

Chinese Journal of Critical Care Medicine

2018, Vol. 38

Issue (3): 246-249 DOI: 10.3969/j.issn.1002-1949.2018.03.016

Effects of combination of mild head hypothermia and IGF-1 on global cerebral ischemia-reperfusion injury in rats

Ao Li, Li Yan-li, Zhang Zhi-qiang, Bian Qing-hu, Liu Zheng, Zhang Shan

Department of Anesthesiology, Second Hospital of Hebei Medical University, Shijiazhuang 050001, China

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Abstract Objective To explore the effects of combination of mild head hypothermia and IGF-1 on global cerebral ischemia-reperfusion injury in rats, and to investigate the mechanism of cerebral protection via PI3K/Akt signaling pathway. Methods Sixty healthy male SD rats weighing 250~280 g were used in this study and randomly divided into five groups (n=12): sham group (group S), global cerebral ischemia-reperfusion injury group (group C), mild head hypothermia group (group H), IGF-1 group (group I), combination of IGF-1 and mild head hypothermia group (group HI). Modified Pulsinelli′s four-vessel occlusion for 15 min was used to establish the model of global cerebral ischemia-reperfusion injury in rats. At the point of 8 h after reperfusion, six rats in each group were used to observe the immunohistochemical expression of pAkt, pFoxO3a, Bcl-2 and Bax protein. The other six rats were killed to observe the expression of pAkt and total Akt protein by Western blot. Results Compared with group S, the expressions of pAkt, pFoxO3a, Bcl-2 and Bax protein were all increased in group C. Compared with group C, the expression of Bax protein was reduced in group H, group I and group HI, while the expressions of pAkt, pFoxO3a, Bcl-2 were significantly increased. Compared with group HI, the expression of pAkt, pFoxO3a, Bcl-2 protein were significantly lower in group H and group I (P<0.05). Compared with group S, the expressions of pAkt protein was higher in group C. Compared with group C, the expression of pAkt protein was higher in group H, group I and group HI. The expression of pAkt protein was significantly highest in group HI (P<0.05). There was no statistically significant difference in the expression of total Akt protein in hippocampus with each group (P>0.05). Conclusion The combination of mild head hypothermia and IGF-1 can alleviate global ischemia-reperfusion injury, and its mechanism is likely to inhibit neuronal apoptosis via upregulating the phosphorylation of Akt and then their substrates.

Key words： Reperfusion injury Phosphoinositide-3-kinase/protein kinase B(PI3K/Akt) Hypothermia Insulin-like growth factor-1(IGF-1) Cerebral protection Apoptosis

Received: 02 January 2018

Corresponding Authors: Zhang Shan, E-mail: zhang18531135269@126.com

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	Ao Li
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Ao Li,Li Yan-li,Zhang Zhi-qiang, et al. Effects of combination of mild head hypothermia and IGF-1 on global cerebral ischemia-reperfusion injury in rats [J]. Chinese Journal of Critical Care Medicine, 2018, 38(3): 246-249.

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http://www.cnemergency.org.cn/EN/10.3969/j.issn.1002-1949.2018.03.016 OR http://www.cnemergency.org.cn/EN/Y2018/V38/I3/246

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