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| MicroRNA-222 modulates the migratory ability of endothelial progenitor cells from human peripheral blood through the Akt signaling pathway
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| Xu Wen-feng, Wang Cui-ping, Zhang Hao, Cai Hua-zhong, Shi Liang, Sun Wen-wen, Ren Guo-qing
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| Department of Emergency, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China
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Abstract Objective To explore the effects of microRNA-222（miR-222） on the migration of endothelial progenitor cells (EPCs) and the role of Akt signaling pathway and its target gene, ETS-1(ETS proto-oncogene 1, transcription factor), in this process. Methods EPCs were respectively isolated, cultured and identified from human peripheral blood. EPCs from healthy donors were transfected with miR-222 mimic, mimic negative control (NC), miR-222 inhibitor, or inhibitor NC. The expression levels of ETS-1 mRNA and miR-222 were measured by qRT-PCR. The protein levels of ETS-1, Akt and p-Akt were analyzed by western blot. The migratory ability of EPCs was assessed using Transwell assay. Results MiR-222 can regulate the expression of ETS-1 mRNA and protein. Over-expression of miR-222 significantly down-regulated the expression of ETS-1 in EPCs at the mRNA and protein levels and inhibited migratory ability of EPCs (all P＜0.05). Furthermore, knockdown of miR-222 significantly up-regulated the expression of ETS-1 in EPCs at the mRNA and protein levels and promoted migratory ability of EPCs (all P＜0.05). Western blot showed that up-regulation of miR-222 reduced the levels of p-Akt. Down-regulation of miR-222 increased the levels of p-Akt (all P＜0.05). Conclusion MiR-222 regulated the migratory ability of EPCs via its target, EST-1, and through Akt signaling pathway.
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Corresponding Authors:
Ren Guo-qing, E-mail: doctorgq@sohu.com
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