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| Effect of ghrelin on gastric juice secretion and gastric blood flow in septic rats |
| Lin Qing-ling, Yin Chao, Zhang Lei, Li Bin, Liu Jian |
| Department of Critical Care Medicine, the First Hospital of Lanzhou University, Lanzhou 730000, China |
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Abstract Objective To investigate the effects of ghrelin on gastric juice secretion and gastric juice pH in septic rats. Methods Seventy-two male Wistar rats were randomly divided into six groups: normal control, sham, sepsis, ghrelin group, growth hormone releasing peptide-6 (GHRP-6) group, and obestatin group, every group have twelve rats. 2 h, 4 h, 8 h and 12 h after the intervention, three rats were sacrificed and the gastric juice was collected to measure the pH value and volume. Results There was no significant difference in pH value and gastric fluid volume between control group and sham operation group (P＞0.05).Compared with sham operation group, the pH value in sepsis group increased significantly at 4 h after intervention (all P＜0.05), and the gastric fluid volume decreased at 2 h, 8 h and 12 h after intervention (all P＜0.05).Compared with sham operation group and sepsis group, the pH value of ghrelin group was significantly lower 4 h after intervention (all P＜0.05), and the gastric juice volume was significantly increased 2 h after intervention (all P＜0.05). Compared with the normal control group, the pH value of GHRP-6 group decreased significantly 2 h after intervention (P＜0.05), and decreased significantly 4 hours after intervention (P＜0.05). Compared with the sepsis group, the pH value of GHRP-6 group decreased significantly 4 h after intervention (P＜0.05). Compared with ghrelin group, the difference was only 8 h after intervention (P＜0.05). The difference was statistically significant (P＜0.05). Compared with sham operation group, sepsis group and ghrelin group, the volume of gastric juice in GHRP-6 group increased significantly 2 h after intervention (P＜0.05). There were significant differences in pH value and gastric fluid volume between obestatin group and other groups (P＜0.05). Conclusion Ghrelin can promote the secretion of gastric juice in septic rats.
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Corresponding Authors:
Li Bin, E-mail: lynd0001@163.com
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