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| The expression of HIF-1α and VEGF in seawater and freshwater aspiration-induced lung injury |
| HUAN Le, DIAO Meng- yuan, SONG Xi, ZHANG Sheng, ZHAO Liang, LIN Zhao- fen |
| Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China |
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Abstract Objective To observe the changes of the expression of hypoxia inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) in seawater and freshwater aspiration-induced lung injury, explore the possible mechanism involved in lung injury. Methods Eighteen rabbits were randomly assigned to 3 groups (6 in each group): the control group, the seawater group and the freshwater group. Rabbits in the latter 2 groups were given 3 mL/kg seawater and 18 mL/kg freshwater intratracheally to induce the models of acute lung injury respectively. Arterial partial oxygen pressure and lung wet/dry weight(W/D) ratio were detected. Protein contents in bronchoalveolar lavage fluid (BALF) and serum,
as well as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 levels in BALF, were also determined. HIF-1α and VEGF expressions were measured via quantitative real-time PCR and Western blot.
Changes in the histopathology of lung specimens were evaluated through hematoxylin-eosin staining and the lung injury scores were calculated by the method of Smith scores. Results Seawater and freshwater aspiration dramatically decreased PaO2/FiO2 in the both groups compared with the control group. Furthermore, the values of TNF-α, IL-6 and IL-1β were significantly increased in BALF. HIF-1α and VEGF expressions were markedly activated, histopathologic changes were more serious and the Smith scores were significantly increased compared with the control group. In addition, seawater aspiration induced dramatically higher values of IL-6, expressions of HIF-1α and VEGF, significantly serious histopathologic changes and higher Smith scores compared with the freshwater group. Conclusion The expression of HIF-1α and VEGF were increased in seawater and freshwater aspiration-induced lung injury.
These results indicate that the HIF-1α/VEGF pathway may play an important role in seawater and freshwater aspiration-induced lung injury. Because of the different mechanisms of seawater and freshwater aspiration-induced lung injury, the HIF-1α/VEGF pathway may have different physiological function in the two forms of lung injury.
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Received: 14 January 2016
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Corresponding Authors:
LIN Zhao-fen，E-mail:linzhaofen@hotmail.com
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