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| Effect of glial glutamate transporter-1 expression in hippocampal CA1 region during global cerebral ischemia reperfusion injury in rats |
| Ma Dong-yang, Li Yan-li, Zhang Zhi-qiang, Bian Qing-hu, Li Ya-nan, An Qi, Zhang Shan |
| Department of Anesthesiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China |
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Abstract Objective To observe the effect of glutamate transporter-1 (GLT-1) dynamic expression of hippocampal CA1 region caused by global cerebral ischemia-reperfusion injury in rats. Methods Ninety male Wistar rats were randomly divided into three groups: control group (group C, n=6): without any treatment. Sham group (group S, n=42): occluded bilateral vertebral arteries and underwent sham operation 48 hours later. Ischemia-reperfusion group (I/R group, n=42): The rat model of global cerebral ischemia-reperfusion was established by four-vessel occlusion. The rats were decapitated in given time points, immediately after ischemia (0 h), 8 h, 16 h, 1 d, 3 d, 5 d, 7 d, and the histological changes of hippocampus and expressions of GLT-1, Bax and Bcl-2 were observed by thionine staining, immunohistochemistry and Western blot techniques. Results Compared with group C, there was no significant difference in histological grade (HG) and neuron density (ND) in hippocampal CA1 region at 7 d ［group C: HG 0, ND: 214±3.7 vs. group S: HG 0-Ⅰ, ND: 206.0±4.6, P>0.05］ while expression of GLT-1, Bcl-2 and Bax in hippocampal CA1 region increased in group S (P<0.05). Compared with group S, HG (Ⅲ) increased and ND (42±3.4) decreased significantly in hippocampal CA1 region in I/R group at 7 d (P<0.05); GLT-1, Bcl-2 expression and Bcl-2/Bax ratio decreased while Bax expression increased (P<0.05). Conclusion Cerebral ischemia-reperfusion injury causes down-regulation of GLT-1 expression in hippocampal CA1 region, which may be one of the mechanisms of ischemia-reperfusion injury.
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Corresponding Authors:
Zhang Shan, E-mail: zhangshan18531135269@126.com
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