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| Comparison on two kinds of spinal cord injury model in mice with self-made spinal impactor device |
| CHEN Xue-zhou, BI Yi-hui, ZHANG Ke-ke, DONG Zhou, ZHANG Ming-kai, YOU Tao |
| The Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China |
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Abstract Objective Comparison of two kinds of spinal cord injury models with the self-made spinal impactor device, in order to establish an improving method. Methods Thirty mice were divided into 3 groups randomly: group A: incision without contusing spinal cord, group B: encroach for thoracic fenestration with contusing method by the self-made spinal device, group C: through intervertebral foramen with contusing method by the self-made spinal device. Intraoperative times, the length of the incision and blood loss were recorded. Basso mouse scale（BMS）, footprint analysis, histology were performed to evaluate spinal cord severity. Results Group C mice showed better results in intraoperative time（36.4±5.21）min, the length of the incision（1.32±0.08）cm and blood loss（0.24±0.05）mL versus those indexes of group B（P＜0.05）. Locomotor testing and histology revealed that the SCI models can
be established via the two methods. Conclusion A simple and stable spinal cord injury free from laminectomy can be made with the self-made device. The method is simpler and can mimic the clinical situation.
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Received: 18 December 2015
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Corresponding Authors:
YOU Tao, E-mail: youtaowh@163.com
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