TGF-β1 在脓毒症炎症反应中的变化及作用：体内及体外研究

doi:doi:10.3969/j.issn.1002-1949.2016.04.005

Chinese Journal of Critical Care Medicine

2016, Vol. 36

Issue (4): 307-311 DOI: doi:10.3969/j.issn.1002-1949.2016.04.005

The effect of TGF-β1 on inflammatory factors in an experimental sepsis model and THP-1 cells

ZHANG Yu，XING Jing，LI Hui-ping

Department of Emergency，the First Affiliated Hospital of Dalian Medical University，Dalian 116011， China

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Abstract Objective This study aimed to investigate the dynamic change of TGF-β1 in serum of a cecal ligation and puncture（CLP）sepsis model in rats，with its effects on inflammatory factors，and discuss the mechanism of TGF-β1 in THP-1 cells. Methods A total of 152 Sprague-Dawley rats were used in vivo experiment. The rats were randomly divided into two groups，including control group（n＝32，four sub-groups，each sub-group 8 rats）and model group（n＝120，four sub-groups，each sub-group 30 rats）. Rats in control groups were sacrificed after anesthesia. Rats in model groups were sacrificed on different time points（6 h，12 h，24 h，48 h）after CLP. The serum was collected to investigate the level of TGF-β1，TNF-α and IL-10 at the same time. Rat's mortality and pathological changes were also investigated. By TGF-β1 siRNA technology，the level of TNF-α and IL-10 were tested in the THP- 1 cell culture medium. Results In model groups，the levels of TGF- β1，TNF- α and IL- 10 were significantly increased at all the time points after CLP（P＜0.05）. The level of TGF-β1 in serum reached the peak at 24 h after CLP. The mortality of rats increased as time gone by. Histopathological re⁃sults in model groups were significantly different from those in the control group. Compared with NC group，cell secretion of TNF-α in THP-1 cells after TGF-β1 RNAi significantly increased（P＜0.05），while the secretion of IL-10 did not increased（P＞0.05）. Conclusion The findings obtained from the experimental sepsis model suggest that TGF- β1 is involved in inflammatory process in sepsis. The findings obtained from the vitro experiment indicates that TNF-α should be promoted by TGF-β1 RNAi in THP-1 cell，while IL-10 should not be affected.

Received: 14 December 2015

Corresponding Authors: XING Jing，E-mail: xingjing0117@163.com

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ZHANG Yu,XING Jing,LI Hui-ping. The effect of TGF-β1 on inflammatory factors in an experimental sepsis model and THP-1 cells[J]. Chinese Journal of Critical Care Medicine, 2016, 36(4): 307-311.

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http://www.cnemergency.org.cn/EN/doi:10.3969/j.issn.1002-1949.2016.04.005 OR http://www.cnemergency.org.cn/EN/Y2016/V36/I4/307

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