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| Expression of NLRP3 inflammasome in renal tissue of rats with acute kidney injury induced by ischemia-reperfusion and intervention of dexamethasone |
| Xuan Xian-fa, Wang Yu-xin, Zhang Yi-qin, Yan Jian-hua, Zhou Li-na, Wu Ting, Zhang Si-yu, Zhao Ben-hua |
| Department of Nephrology, the Second Affiliated Hospital of Xiamen Medical College (the Second Hospital of Xiamen), Xiamen 361021, China |
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Abstract Objective To investigate the expression of NLRP3 inflammasome and the effect of dexamethasone in renal tissue of rats with acute kidney injury induced by ischemia-reperfusion. Methods Twenty-one normal male SD rats were randomly divided into three groups, respectively ischemia-reperfusion group (Group A), sham operation group (Group B) and dexamethasone + ischemia reperfusion group (Group C). The differences of serum creatinine, urea nitrogen and NLRP3 expression among the groups were compared. The effect of dexamethasone on the content of NLRP3 inflammatory body in rats was analyzed. The expression of NLRP3 protein in rat renal tissue was detected by Western blot; the levels of IL-1β and IL-18 in rat kidney were detected by ELISA; the expression of NLRP3 in kidney was detected by immunohistochemistry, renal tubulointerstitial injury was detected by pathological examination. Results The levels of serum creatinine, urea nitrogen, IL-1β, IL-18 and NLRP3 inflammasome protein in group A rats were significantly higher than those in group B ［(89.5±10.8) μmol/L & (22.3±3.1) μmol/L, (23.1±5.3) mmol/L & (4.8±0.7) mmol/L, (0.39±0.11) & (0.08±0.01), (21.7±5.6) pg/mL & (5.7±1.9) pg/mL, (42.5±6.7) pg/mL & (11.4±2.3) pg/mL; P<0.05］. The levels of serum creatinine, urea nitrogen, IL-1β, IL-18 and NLRP3 inflammasome protein in group C were significantly lower than those in group A ［(53.4±8.3) μmol/L & (89.5±10.8) μmol/L, (9.6±2.9) mmol/L & (23.1±5.3) mmol/L, (0.21±0.07) & (0.39±0.11); P<0.05］. Immunohistochemistry showed that the expression of NLRP3 in renal tubular epithelial cells in rats with acute kidney injury after ischemia-reperfusion was significantly increased. Pathological examination showed that the tubulointerstitial injury was severe in the ischemia-reperfusion group. Conclusion Acute kidney injury after ischemia-reperfusion is associated with an increase in serum creatinine, IL-1β, IL-18 and NLRP3 inflammasome levels and NLRP3 expression of renal tissue. Intervention with dexamethasone can reduce the NLRP3 inflammasome content in renal tissue and reduce the degree of damage to renal tissue.
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Corresponding Authors:
Department of Nephrology, the Second Affiliated Hospital of Xiamen Medical College (the Second Hospital of Xiamen), Xiamen 361021, China
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