硫酸镁对百草枯中毒肺损伤后大鼠环氧化酶-2和钙-ATP酶表达及胶原生成的实验研究

doi:10.3969/j.issn.1002-1949.2017.11.018

Chinese Journal of Critical Care Medicine

2017, Vol. 37

Issue (11): 1045-1048 DOI: 10.3969/j.issn.1002-1949.2017.11.018

The expression of COX-2， Ca2+-ATPase and collagenation in paraquat-induced lung injury rats treated with magnesium sulfate

Li Jun-ying, Liang Zong-an, He Qing, Zheng Jun

Department of Emergency Intensive Care Unit, Sichuan Provincial People′s Hospital, Chengdu 610072, China

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Abstract Objective To investigate the expressions of COX-2， Ca2+-ATPase and collagenation in paraquat-induced lung injury in rats treated with magnesium sulfate. Methods Sixty male Sprague-Dawley rats were randomly (random number method) divided into three different groups: paraquat intoxication group (PQ group); magnesium sulfate group (MS group); controlled group (NS group). PQ group and MS group were given paraquat through intra-gastric administration (80 mg/kg). MS group was intraperitoneally administrated given douched magnesium sulfate (30 mg/kg per day), while NS group was given equivalent volume of normal saline. The rats of these three groups were further divided into four sub-groups: 3 d, 7 d, 14 d and 21 d. The level activity of Ca2+-ATPase in lung homogenate was measured. The expressions of COX-2 and collagen fiber were detected by immunohistochemistry. Results The COX-2 expression was significantly higher in the rats lung of paraquat intoxication groups (PQ group and MS group) compared with NS group, while there was a significant difference between MS group and PQ group (P<0.05). The Ca2+-ATPase activity in PQ group was lower than that of MS group(P<0.05). Compared with NS group, the Ca2+-ATPase activity of PQ group was significantly different during treatment period (P<0.01), but the Ca2+-ATPase activity of MS group was significantly different on the 3rd, 7th and 21th day (P<0.05). In MS group, the Ca2+-ATPase activity increased from the 14th day and returned to normal level on the 21th day. However, the Ca2+-ATPase activity of PQ group did not increase until on the 21th day. The collagen fiber among these groups had no significant difference on the 3rd day. But a gradually increase of collagenation could be observed in PQ group on the 14th day, and it presented a strongly fibrosis. MS group had lower collagen fiber hyperplasia and structure destroy than PQ group. Conclusion Magnesium sulfate could lessen lung injury by reducing the expression of COX-2, decreasing angiogenesis and inhibiting calcium overload.

Received: 28 May 2017

Corresponding Authors: Liang Zong-an, E-mail:Liang.zongan@163.com

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Li Jun-ying,Liang Zong-an,He Qing, et al. The expression of COX-2， Ca2+-ATPase and collagenation in paraquat-induced lung injury rats treated with magnesium sulfate[J]. Chinese Journal of Critical Care Medicine, 2017, 37(11): 1045-1048.

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http://www.cnemergency.org.cn/EN/10.3969/j.issn.1002-1949.2017.11.018 OR http://www.cnemergency.org.cn/EN/Y2017/V37/I11/1045

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