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| Changes of bacterial translocation and composition of intestinal flora in different stages of severe acute pancreatitis |
| Man Yi, Chang Jia-wei, Tang Qin-qing |
| Department of Emergency Medicine, the First Affiliated Hospital of Anliui Medical University, Hefei 230032, China |
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Abstract Objective This paper is to explore changes of intestinal flora and bacterial translocation in rats with severe acute pancreatitis (SAP). Methods The healthy SD rats were randomly divided into Control group (n=10) and SAP group (n=30). The SAP group was divided into 6, 12 and 24 h subgroups, with 10 rats in each subgroup.The model of severe acute pancreatitis of rats was induced by injecting adversely 5% sodium taurocholate into the common billiary-pancreatic duct. The rats were sacrificed after 6, 12 and 24 h respectively. The serum amylase and endotoxin level were detected and the pathological changes of the pancreas and intestinal mucosa were observed. Bacterial translocation rate in abdominal cavity and viscera was detected by bacterial culture, and positive colonies were identified by mass spectrometry. 16S rDNA and real-time fluorescence quantitative polymerase chain reaction were used to quantitative analysis of enteric bacteria. Results The serum amylase(U/L) in the SAP group was significantly higher than that in the control group and more than three times（Control group=2086±686,6 h=8707±1672,12 h=9012±1505,24 h=8797±1298;P<0.01). The pancreatic tissue and the small intestinal mucosa showed obvious pathological damage. As the course of the disease prolonged, the intestinal Escherichia coli(×10-5) in the SAP group continued to proliferate (Control group=4.53±1.26,6 h=10.07±2.57,12 h=16.18±3.51,24 h=19.45±4.70;P<0.01), the lactobacillas(×10-3) continues to decreased (Control group=23.12±4.46,6 h=11.43±2.68,12 h=5.20±2.07,24 h=2.02±0.94;P<0.01), the bifidobacterium(×10-5) also continues to decrease(Control group=23.30±6.00,6 h=17.61±3.94,12 h=13.21±2.84,24 h=9.49±3.41;P<0.01) and the positive rate of serum endotoxin test, the mesenteric lymph node and the bacterial translocation rate of abdominal viscera increased(serun endotoxin:Control group=0,6 h=30%,12 h=50%,24 h=60%; liver:Control group=0,6 h=20%,12 h=50%,24 h=70%;pancreas:Control group=0,6 h=30%,12 h=40%,24 h=60%;mesenteric lymph nodes:Control group=0,6 h=40%,12 h=70%,24 h=80%;P<0.01). Conclusion In rats with severe acute pancreatitis, the intestinal mucosal barrier is damaged, the intestinal flora is disturbed, the proportion of aerobic bacteria and anaerobic bacteria in the intestinal cavity is inverted. As the course of the disease is prolonged, the pathogenic bacteria of Escherichia coli are increasing, and the anaerobic probiotics, such as Lactobacillus and Bifidobacterium, are constantly decreasing, causing intestinal bacteria and endotoxin. There is a certain relationship between displacement and SAP and concurrent pancreas and systemic infection.
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Corresponding Authors:
Tang Qin-qing, E-mail: tangqinqing@aliyun.com
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