生长激素释放肽对失血性休克大鼠心肌保护作用及对核因子-κB的影响

doi:10.3969/j.issn.1002-1949.2019.01.014

Chinese Journal of Critical Care Medicine

2019, Vol. 39

Issue (1): 66-70 DOI: 10.3969/j.issn.1002-1949.2019.01.014

Effect of exogenous ghrelin on myocardial protection and NF-κB in rats after hemorrhagic shock

Zhang Li-na, Yue Zi-yong

Department of Anesthesiology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China

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Abstract Objective To investigate the effect and the underlying mechanism of exogenous ghrelin on myocardial protection in rats after hemorrhagic shock(HS). Methods Thirty two male Sprague-Dawley (SD) rats weighing 300～350 g were randomly divided into four groups (n=8 per group): sham operation group (sham group), sham operation plus ghrelin group (sham+ghrelin group), HS group, HS plus ghrelin group (HS+ghrelin group). HS was induced in male SD rats by withdrawing blood to a mean arterial pressure (MAP) of 40 mm Hg for 1 h, rats were then received ghrelin (10 nmol/kg) or vehicle intravenously and resuscitated with the shed blood and equal Ringer Lactate solution followed by an observation for 2 h, with blood gas analysis and hemodynamic monitoring. After resuscitation, samples were collected and analyzed for myocardial histopathology, malondialdehyde (MDA)content, superoxide dismutase(SOD) activity, myeloperoxidase (MPO) activity, and plasma inflammatory cytokines (TNF-α and IL-6), expression of nuclear NF-κB were also evaluated. Results Ghrelin alleviated decreased MAP after resuscitation compared with HS rats at both time points following resuscitation(P<0.05). Compared with the two sham groups, myocardial injury, MDA content, MPO activity, plasma TNF-α and IL-6 levels, NF-κB activation from HS rats were significantly increased and SOD activity was lower than sham group(P<0.05). After administration of ghrelin, those parameters analyzed were lower than those without ghrelin in HS rats［［MDA：（2.86±0.08）nmol/mg prot vs.(1.45±0.29)nmol/mg prot，MPO：（2.23±0.20）U/g prot vs. (1.87±0.22)U/g prot, TNF-α:(62.71±4.31)pg/mL vs. (35.16±4.06)pg/mL, IL-6:(73.33±13.94)pg/mL vs.(42.22±7.92)pg/mL, P<0.05］; SOD activity of HS+ghrelin group was significantly higher than that of HS group［(72.77±4.90)U/mg prot vs. (89.31±7.35)U/mg prot, P<0.05］. Conclusion Exogenous ghrelin attenuates myocardial injury after hemorrhagic shock, and these beneficial effects of ghrelin appear to be mediated through inhibition the NF-κB signaling pathway. 

Key words： Hemorrhagic shock Myocardial protection Ghrelin NF-κB

Corresponding Authors: Yue Zi-yong, E-mail: yueziyong@126.com

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Zhang Li-na,Yue Zi-yong. Effect of exogenous ghrelin on myocardial protection and NF-κB in rats after hemorrhagic shock[J]. Chinese Journal of Critical Care Medicine, 2019, 39(1): 66-70.

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http://www.cnemergency.org.cn/EN/10.3969/j.issn.1002-1949.2019.01.014 OR http://www.cnemergency.org.cn/EN/Y2019/V39/I1/66

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