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| Effect of fingolimod on learning and memory capacity in a mouse model of traumatic brain injury |
| Wang Wei, Feng Zhu-sheng, Yin Wen |
| Department of Emergency, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China |
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Abstract Objective To investigate the effect of fingolimod（FTY-720）on learning and mem⁃ ory capacity after traumatic brain injury（TBI）. Methods Forty-eight C57BL/6 mice were randomly di⁃ vided into sham group, TBI group and FTY- 720 treated group（TBI+FTY group）. TBI model was in⁃ duced by control cortical injury device; rats in TBI+FTY group were administrated with FTY-720 intra⁃ peritoneally following TBI. Expression of cleaved caspase-3 and B cell lymphoma（Bcl-2）in hippocam⁃ pus was determined by Western-blotting. The learning and memory capacity was evaluated by Morris wa⁃ ter maze（MWM）test. Results Compared to sham group, the expression of cleaved caspase-3 was in⁃ creased and the Bcl-2 level was reduced in TBI group. In addition, the escape latency was prolonged and the times of platform crossing and duration in target quadrant were decreased after TBI. Administration of FTY-720 could not only reverse the cleaved caspase-3 and Bcl-2 expression, but also prevent the al⁃ terations of escape latency, platform crossing times and duration in target quadrant induced by TBI. Conclusion TBI-triggered hippocampal cellular apoptosis and cognitive dysfunction could be effectively rescued by FTY-720.
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Received: 17 May 2016
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Corresponding Authors:
Yin Wen, E-mail: xjyyyw@126.com
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