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| Propofol improves gastric mucosal blood flow and nitric oxide/endothelin-1 balance and alleviates gastric mucosal injury after trauma hemorrhagic shock resuscitation in rabbits |
| Niu Jing-hui, Qi Qi, Zhang Li-feng, Wang Fei, Lv Yan-xia |
| Department of Orthopedics, the Third Affiliated Hospital of Hebei Medical University, Shijiazhuang 050051, China |
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Abstract Objective To investigate the effects of propofol on gastric mucosal blood flow(GMBF), nitric oxide(NO) and endothelin(ET), and to confirm the role and mechanism of propofol in the gastric mucosal injury after trauma hemorrhagic shock resuscitation in rabbits. Methods Seventy-five healthy adult male rabbits were randomly divided into 5 groups of 15 rabbits: control(S group), trauma hemorrhagic shock resuscitation gastric mucosal injury(M group) and 10 min before hemorrhage(P1 group), 10 min before resuscitation(P2 group), 20 min after resuscitation(P3 group) application propofol group ［5 mg/kg propofol was injected intravenously, then 20 mg/(kg·h) was infused］. The S and M groups were given the same amount of saline. The GMBF was detected by laser Doppler flowmetry. The gastric tissue samples were obtained at 90 min after resuscitation. Gastric mucosal damage index(DI) was calculated by Guth method. The contents of NO and ET-1 were determined by nitrate reductase and radioimmunoassay respectively. The NO/ET-1 ratio was calculated and the correlation between GMBF and NO/ET-1 ratio and between DI value and GMBF, NO, ET-1 or NO/ET-1 ratio was observed. Results Compared with group S, the GMBF, NO and NO/ET-1 ratio were significantly lower, while DI and ET-1 were significantly high after resuscitation in group M and group P(P＜0.05 or P＜0.01). ②The GMBF at 30 min and 90 min after resuscitation in group P1, P2 and that at 90 min after resuscitation in group P3 were significantly higher, and NO content and NO/ET-1 ratio in group P1, P2 or P3 were significantly higher, but DI and ET-1 content in group P1, P2 or P3 were significantly lower than those in group M(P＜0.05 or P＜0.01). ③The GMBF at 90 min after resuscitation and NO/ET-1 ratio were significantly higher, but DI was significantly lower in group P1 than in group P3(P＜0.05 or P＜0.01). ④At 90 min after resuscitation, there was a close correlation between GMBF and NO/ET-1, DI and GMBF, NO or NO/ET-1, DI and ET-1(r=0.913, -0.699, -0.762, -0.678 and 0.705 respectively, P＜0.01). Conclusion Propofol can improve NO and ET-1 balance, raise NO/ET-1 ratio and is beneficial to maintaining GMBF. Propofol can also reduce the gastric mucosal injury after trauma hemorrhagic shock resuscitation in rabbits; it is of great effectiveness when being applied before ischemia.
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Received: 29 December 2016
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Corresponding Authors:
Lv Yan-xia, E-mail: lyxshsy@163.com
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Address: 1 North 2nd Street,
Zhongguancun, 