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| The role of IL-33 in a murine model of renal ischemia and reperfusion injury |
| Zhu Ping，Xie Li，Dong Chuan-jiang，Huang Wen-feng，Zhang Xiang-wen，Yang Lin |
| Department of Renal Division in In?ternal Medicine，the First College of Clinical Medical Science，China Three Gorges University，Yichang 443002，China |
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Abstract Objective To explore the role of IL-33 in a murine model of renal ischemia and re⁃ perfusion injury. Methods Forty-five male C57BL/6 mice were divided into three groups: sham group （sham）; ischemia and reperfusion injury group（PBS + I/R）; IL- 33 + ischemia and reperfusion injury group（rIL-33+I/R）. In sham mice，an abdominal incision was made，followed by dissection of the bilat⁃ eral renal pedicle，with no further cross-clamping of arteries. PBS+I/R mice were administered（i. g. ） phosphate buffered solution 1 hour before surgery whereas rIL-33 + I/R mice were administered rIL-33 5 μg 1 hour before surgery. Mice in NS+I/R and rIL-33 + I/R groups were subjected to renal ischemia to induce acute kidney injury after 45-min clamping of bilateral renal arteries. Serum levels of creatinine and blood urea nitrogen levels were measured. H&E staining was undertaken to detect damaged renal tis⁃ sue. Myeloperoxidase（MPO）activity and immunofluorescent detection of IL-33 expression was under⁃taken for each group. Level of IL-17A was monitored by ELISA. Results The expression of rIL-33 in murine renal was increased during renal ischemia and reperfusion injury. Serum creatinine and blood urea nitrogen levels were elevated in PBS+ I/R and rIL-33+I/R groups compared with sham mice（P＜ 0.05），whereas those in the PBS+I/R group were significantly higher compared with the rIL- 33 + I/R group（P＜0.05）. The damage of renal tissue and neutrophils infiltration in rIL- 33 + I/R groups were significantly lower than in PBS+I/R mice. The MPO activity and level of IL-17A in rIL-33+I/R was re⁃ duced than that in PBS+I/R groups（P＜0.05）. Conclusion IL-33 can inhibit renal I/R injury by in⁃ hibiting release of IL-17A and neutrophils infiltration.
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Received: 19 January 2016
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| About author:: Zhu Ping，E-mail: topgan2000@163.com |
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