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| Influence on Th17/Treg cells' balance in peripheral blood in patients with severe acute pancreatitis by early peritoneal lavage |
| Ma Zhen, Su Yu |
| Department of General Surgery, Xiantao First People's Hospital, Xiantao 433000, China |
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Abstract Objective To explore the influence on Th17/Treg cells' balance in peripheral blood in patients with severe acute pancreatitis（SAP）by early peritoneal lavage, to provide reference for its clinical treatment. Methods A total of 132 cases with SAP were selected, and were randomly divided into group A and group B, 66 cases per group. Sixty healthy volunteers in the same period were selected by matching as control group. Patients in group A were given comprehensive treatments at the early stage, and patients in group B were treated by peritoneal lavage and drainage on the basis of group A. Th17 and Treg cells proportion in peripheral blood were detected in control group on the day of physical examination, and on the first, forth, seventh day after admission in group A and B, and Th17/Treg were calculated. Serum amylase, C reactive protein（CRP）and APACHEⅡ scores on the first, forth, seventh day after admission in group A and B were compared, SIRS' amount and duration, also MODS' amount in 72 h in group A and B were noted. Results Th17 and Treg cells proportion, Th17/Treg ratio on the first day after admission in group A and B were all higher than that in control group on the day of physi⁃ cal examination（t＝3.92～7.39, P＜0.05 or P＜0.01）. After treatment, except Treg proportion and Th17/Treg ratio on the seventh day after admission in group B, Th17, Th17/Treg ratio, APACHE Ⅱ score, serum amylase, CRP levels in group A and B were all decreased successively（t＝3.40～7.17, P＜ 0.05 or P＜0.01）, yet Treg cells proportion was all increased（t＝3.56～6.25, P＜0.05 or P＜0.01）, and Th17 and Treg cells proportion, Th17/Treg ratio, APACHEⅡ score, serum amylase, CRP levels in group B were all lower than that in group A（t＝3.55～7.02, P＜0.05 or P＜0.01）. Rates of SIRS and MODS in 72 h in group B were both lower than that in group A（χ2＝ 4.13, 4.59, P＜0.05）, and SIRS du⁃ ration and hospitalization time in group B were both less than that in group A（t＝5.45, 4.29, P＜0.05）. Spearman correlation analysis showed that Th17/Treg ratio was significantly positively correlated with CRP in patients with SAP（r＝0.785, P＜0.05）. Conclusion Early peritoneal lavage eliminates intraabdominal inflammatory mediators, reduces SAP patients' early acute inflammatory reaction, promotes the Th17/Treg balance, blocks or reduces the development of SIRS, reduces MODS' incidence, shortens hospitalization time, so it has certain guidance and reference significance for SAP's clinical treatment.
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Received: 09 March 2016
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| About author:: Ma Zhen, E-mail: mazhensuyu@sina.com |
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Address: 1 North 2nd Street,
Zhongguancun, 