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| Effect of curcumin pretreatment on heat stroke rat′s proteins related to intestinal mucosa and serum inflammatory factors in dry-heat environment |
| Dong Xiang, Xu Qin, Gao Xiao-kang, Li Jia-jia, Tang Jun, Kang Yan, Shen Cai-fu, Liu Jiang-wei |
| Key Laboratory of the Special Environment Medicine of Xinjiang, General Hospital of Xinjiang Military Command, Urumqi 830000, China |
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Abstract Objective To investigate the effects of curcumin pretreatment on the expressions of HSP70, HMGB1 and ICAM-1 in heat stroke rat′s intestinal mucosa and serum inflammatory factors level in dry-heat environment. Methods 30 SD rats were randomly divided into normal control group (NC group), dry heat group (DH group) and curcumin pretreatment group (Cur group), with 10 rats in each group. NC and DH groups were gavaged with saline. Cur group was gavaged with 200 mg/kg curcumin, once a day for 7 days. After the end of gavage, DH and Cur groups were placed in the experimental cabin of the simulated dry-heat environment, temperature of (41±0.5)℃, relative humidity of (10±1)%, exposed 150 min to cause severe heatstroke model. The expression of HSP70, HMGB1 and ICAM-1 protein were detected by Western blotting in intestinal mucosa, the concentration of LPS, TNF-α, IL-1β and IL-6 were detected by ELISA method. Results The expressions of HSP70, HMGB1, ICAM-1 and the concentration of LPS, TNF-α, IL-1β and IL-6 significantly increased in DH group campared with NC group (P<0.05). The expressions of HSP70 significantly increased in Cur group (8.75±0.46) compared with DH group (2.17±0.14)，P<0.05. The expressions of HMGB1, ICAM-1 significantly decreased in Cur group (6.95±0.42, 9.31±0.76) compared with DH group (17.76±1.54, 19.39±1.64)，P<0.05, the concentration of LPS, TNF-α, IL-1β, IL-6 significantly decreased in Cur group compared with DH group. Conclusion Curcumin pretreatment may protect intestinal mucosa and reduce the migration of bacteria or toxins in intestinal cavity by regulating the expression of HSP70, HMGB1 and ICAM-1, thereby alleviating systemic inflammatory response caused by heatstroke in dry-heat environment.
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Corresponding Authors:
Liu Jiang-wei, E-mail: ljw273273@163.com
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| ［1］Liu Z, Sun X, Tang J, et al. Intestinal inflammation and tissue injury in response to heat stress and cooling treatment in mice［J］. Mol Med Rep, 2011, 4(3): 437-443.
［2］Yi G, Li L, Luo M, et al. Heat stress induces intestinal injury through lysosome-and mitochondria-dependent pathway in vivo and in vitro［J］. Oncotarget, 2017, 8(25): 40 741-40 755.
［3］Pearce SC, Sanz-Fernandez MV, Hollis JH, et al. Short-term exposure to heat stress attenuates appetite and intestinal integrity in growing pigs［J］. J Anim Sci, 2014, 92(12): 5444-5454.
［4］Vecchi Brumatti L, Marcuzzi A, Tricarico PM, et al. Curcumin and inflammatory bowel disease: potential and limits of innovative treatments［J］. Molecules, 2014, 19(12): 21 127-21 153.
［5］ou Zhou R, Liu JW, Zhang D, et al. Heatstroke model for desert dry-heat envionment and observed organ damage［J］. Am J Emerg Med, 2014, 32(6): 573-579.
［6］李佳佳，刘江伟，姚刚，等. 姜黄素对沙漠干热环境大鼠生存率影响的实验［J］. 中国比较医学杂志， 2015， 25(10): 24-28.
［7］Wang LC, Chang CP, Chio CC, et al. Hypobaric hypoxia preconditioning attenuates experimental heatstroke syndromes via preinduction of heat shock protein 70［J］. Am J Med Sci, 2012, 344(5): 383-390.
［8］Liu Y, Wang Z, Xie W, et al. Oxidative stress regulates mitogen-activated protein kinases and c-Jun activation involved in heat stress and lipopolysaccharide-induced intestinal epithelial cell apoptosis［J］. Mol Med Rep, 2017, 16(3): 2579-2587.
［9］孙杰，陈蕊，董家辉, 等. 体外培养人肠上皮细胞株接受脂多糖联合热打击后白细胞介素6、肿瘤坏死因子α的释放［J］. 中国组织工程研究， 2014， 18(51): 8238-8242.
［10］Tang J, Deng P, Jiang Y, et al. Role of HMGB1 in propofol protection of rat intestinal epithelial cells injured by heat shock［J］. Cell Biol Int, 2013, 37(3): 262-266.
［11］Sumagin R, Brazil JC, Nava P, et al. Neutrophil interactions with epithelial-expressed ICAM-1 enhances intestinal mucosal wound healing［J］. Mucosal Immunol, 2016, 9(5): 1151-1162.
［12］Sumagin R, Robin AZ, Nusrat A, et al. Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment［J］. Mucosal Immunol, 2014, 7(4): 905-915.
［13］Lambert GP. Role of gastrointestinal permeability in exertional heatstroke［J］. Exerc Sport Sci Rev, 2004, 32(4): 185-190.
［14］Petrof EO, Ciancio MJ, Chang EB. Role and regulation of intestinal epithelial heat shock proteins in health and disease［J］. Chin J Dig Dis, 2004, 5(2): 45-50.
［15］Chang CH, Kao CH, Chio CC, et al. Attenuating heatstroke-induced acute lung inflammation, edema, and injury in rats by exercise preconditioning［J］. J Trauma Acute Care Surq, 2013, 74(4): 1052-1059.
［16］王丹妹，何佟，吉丽敏，等. 全身热应激预处理对大鼠肠缺血-再灌注损伤程度的影响及机制［J］. 山东医药， 2012， 52(38): 14-16.
［17］Zheng Z, Kim JY, Ma H, et al. Anti-inflammatory effects of the 70 kDa heat shock protein in experimental stroke［J］. J Cereb Blood Flow Metab, 2008, 28(1): 53-63.
［18］姚庆华，林妙，汪玉琪，等. 氧化应激在姜黄素诱导肺癌细胞凋亡中的作用及机制研究［J］. 中华中医药杂志， 2016， 31(2): 614-618
［19］Angelo LS, Maxwell DS, Wu JY, et al. Binding partners for curcumin in human schwannoma cells: biologic implications［J］. Bioorg Med Chem, 2013, 21(4): 932-939.
［20］Leon LR, Dineen S, Blaha MD. Attenuated thermoregulatory, metabolic, and liver acute phase protein response to heat stroke in TNF receptor knockout mice［J］. Am J Physiol Regul Integr Comp Physiol, 2013, 305(12): R1421-1432.
［21］Hammami MM, Bouchama A, Al-Sedairy, et al. Concentrations of soluble tumor necrosis factor and interleukin-6 receptors in heatstroke and heatstress［J］. Crit Care Med, 1997, 25(8): 1314-1319.
［22］Phillips NA, Welc SS, Wallet SM, et al. Protection of intestinal injury during heat stroke in mice by interleukin-6 pretreatment［J］. J Physiol, 2015, 593(3): 739-752.
［23］Silva LS, Catalao CH, Felippotti TT, et al. Curcumin suppresses inflammatory cytokines and heat shock protein 70 release and improves metabolic parameters during experimental sepsis［J］. Pharm Biol, 2017, 55(1): 269-276. |
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