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| Evaluation of glycogen synthase kinase-3β in the diagnosis and prognosis of sepsis and related acute kidney injury |
| Zheng Wen-bo |
| Department of Severe Medicine, Chengdong Branch of Fujian Quanzhou First Hospital, Quanzhou 362000, China |
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Abstract Objective To evaluate the diagnostic value of glycogen synthase kinase-3β (GSK-3β) in sepsis and related acute kidney injury(AKI) and its prognostic value. Methods 104 patients with sepsis treated in our hospital from September 2016 to August 2017 were divided into two groups: severe sepsis group (70 cases) and normal sepsis group (34 cases), and another 100 healthy subjects were selected as the control group during the same period. The clinical data of the three groups were collected. Blood and urine samples were collected within 24 hours after instillation. Serum and urinary GSK-3β levels were detected by sandwich enzyme immunoassay (ELISA). Results Compared with the control group, the relative expression levels of GSK-3β in serum and urine of patients with severe sepsis and normal sepsis was higher (P<0.05). Severe sepsis patients showed higher serum level of GSK-3β than sepsis patients (2.42±1.15 vs. 1.69±0.72，P<0.05). However, there was no significant difference in urine level of GSK-3β in the two groups of patients (1.59±0.67 vs. 1.41±0.53, P>0.05). The Pearson results showed that the serum GSK-3β was positively correlated with urinary GSK-3β, leukocyte count and procalcitonin (P<0.05), while urine GSK-3β was positively correlated with glomerular filtration rate (P<0.05), but was negatively correlated with serum creatinine and APACHE Ⅱ score (P<0.05). The relative expression of GSK-3β in serum and urine of patients with severe sepsis subgroup was higher than that of the improved subgroup and healthy control group (P<0.05). The relative expression levels of GSK-3β in blood and urine samples of patients with AKI and non-AKI subgroup were higher than those of the control group (P<0.05). The relative expression of blood GSK-3β was higher in patients with severe AKI than that in patients with non-AKI(P<0.05). The relative expression of GSK-3β in blood and urine of patients with severe sepsis-aggravated AKI was higher than that of the improved subgroup and healthy control group (P<0.05), and the difference was statistically significant (P<0.05). Conclusion GSK-3β is highly expressed in blood and urine samples of patients with sepsis and related AKI, which plays an important role in the clinical evaluation of the severity of the disease and the prognosis of patients with sepsis and related AKI.
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| About author:: Zheng Wen-bo, E-mail: zwenbo03@163.com |
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