脑肠肽对脓毒症大鼠胃液分泌及胃血流的影响

doi:10.3969/j.issn.1002-1949.2019.11.014

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摘要目的观察脑肠肽（Ghrelin）对脓毒症大鼠胃液分泌量、胃液pH值及胃血流的影响。方法将72只雄性Wistar大鼠按随机数字表法分为六组，正常对照组、假手术组、脓毒症组、Ghrelin组、促生长激素释放肽-6(GHRP-6)组及Obestatin组（每组12只），各组于造模成功并给予干预措施后测胃血流，并于干预后2、4、8、12 h分别处死3只大鼠，取胃，收集胃液测量胃液的pH值及胃液的体积。结果脓毒症组胃大弯处血流较正常对照组明显减少(284.31±95.66 vs. 338.13±4.70，P﹤0.05），Ghrelin组血流较脓毒症组明显增多（377.83±99.01 vs. 284.31±95.66，P<0.05），Obestatin组血流较Ghrelin组明显减少（320.68±130.06 vs. 377.83±99.01，P﹤0.05）；正常对照组与假手术组各时间点pH值及胃液体积比较差异均无统计学意义（P＞0.05）。与假手术组比较，脓毒症组pH值在干预后4 h起即明显升高（均P＜0.05），胃液体积在干预后2、8、12 h减小（均P＜0.05）。与假手术组和脓毒症组比较，Ghrelin组和GHRP组pH值在干预后4 h起即明显降低（均P＜0.05），Ghrelin组胃液体积在干预后2 h即明显升高（均P＜0.05）。与Ghrelin组比较，GHRP组pH值仅在干预后8 h时间点差异有统计学意义（P＜0.05）；GHRP-6组与假手术组、脓毒症组及Ghrelin组比较，胃液体积在干预后2 h起即明显升高（P＜0.05）。Obestatin组与其余各组比较，pH值及胃液体积在各时间点差异均有统计学意义（均P＜0.05）。结论 Ghrelin能够改善脓毒症大鼠胃血流，促进脓毒症大鼠胃液的分泌。

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	林庆玲
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	刘健

关键词 ：脑肠肽（Ghrelin）, 促生长激素释放肽-6(GHRP-6), 脓毒症, pH, 胃液分泌量

基金资助:甘肃省自然科学基金项目(1308RJZA240)；兰州大学第一医院院内基金项目（ldyyyn2014-07);兰州市城关区科技发展计划项目（2017SHFZ0038）

通讯作者: 李斌（1972-），男，主任医师，E-mail:lynd0001@163.com。

作者简介: 林庆玲（1985-），女，硕士，主治医师，E-mail:qingling0633@163.com。

引用本文:

林庆玲，尹超，张磊，李斌，刘健. 脑肠肽对脓毒症大鼠胃液分泌及胃血流的影响[J]. 中国急救医学, 2019, 39(11): 1080-1083.
Lin Qing-ling, Yin Chao, Zhang Lei, Li Bin, Liu Jian. Effect of ghrelin on gastric juice secretion and gastric blood flow in septic rats. Chinese Journal of Critical Care Medicine, 2019, 39(11): 1080-1083.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2019.11.014 或 http://www.cnemergency.org.cn/CN/Y2019/V39/I11/1080

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