预先应用甲状腺素片对脓毒症大鼠肿瘤坏子因子-α和肺组织病理的影响

doi:10.3969/j.issn.1002-1949.2018.02.018

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摘要目的探讨预先应用甲状腺素片对脓毒症大鼠肿瘤坏死因子-α （TNF-α）和肺组织病理的影响。方法大鼠尾静脉注射脂多糖（LPS，5 mg/kg）建立脓毒症模型。36只雄性Wistar大鼠随机分为空白对照组（C组）、脓毒症组（S组）、甲状腺素预先干预组（L组），每组12只。L组预先左旋甲状腺素钠（10 μg/100 g）灌胃7 d，S组和C组等量生理盐水灌胃7 d。建模后0、3、6、12、24 h采血，测血清TNF-α、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）水平，24 h处死大鼠并取肺组织，测肺组织湿/干质量比（W/D），行HE染色并免疫组化。结果 FT3、FT4在0 h各组均无变化（P＞0.05），S组、L组3 h降低，6 h最低，然后逐渐上升，在3、6、12、24 h等时间点较C组明显降低（P＜0.01）,而在3、6、12、24 h时间点L组较S组明显升高（P＜0.01）。S组和L组W/D较C组升高（P＜0.01），较S组升高更明显（P＜0.01）。病理显示，C组肺泡结构完整；S组肺泡壁塌陷，间隔增宽，炎细胞浸润明显；L组较S组减轻。血清TNF-α在S组和L组3 h升高，6 h达峰值，较C组升高（P＜0.01），较S组升高更明显（P＜0.01）；肺组织TNF-α 阳性细胞数量和染色强度S组最多、最强，L组次之，C组最少、最弱。〖HTH〗结论〓〖HTSS〗预先应用甲状腺素片能降低脓毒症大鼠血清TNF-α水平，改善肺组织病理改变。

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	邹文茂
	李景辉
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	敖雪

收稿日期: 2017-09-20

基金资助:海口医院重点培育学科（海市医字［2014］70号）

通讯作者: 李景辉（1966-），男，教授，主任医师，硕士生导师，E-mail:shuo7076@163.com。

作者简介: 邹文茂（1984-），男，硕士研究生，住院医师，E-mail:zwmsci@163.com。

引用本文:

邹文茂，李景辉，刘蕾，刘芙蓉，邓超，敖雪. 预先应用甲状腺素片对脓毒症大鼠肿瘤坏子因子-α和肺组织病理的影响[J]. 中国急救医学, 2018, 38(2): 179-183.
Zou Wen-mao, Li Jing-hui, Liu Lei, Liu Fu-rong, Deng Chao, Ao Xue. Effect of thyroxine pretreatment on tumor necrosis factor-α and lung pathology in septic rats. Chinese Journal of Critical Care Medicine, 2018, 38(2): 179-183.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.02.018 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I2/179

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