重组降钙素原免疫小鼠对脓毒症诱导的急性肾损伤的保护作用

doi:10.3969/j.issn.1002-1949.2018.02.008

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摘要目的了解重组降钙素原（recombinant procalcitonin, rPCT）免疫小鼠对脓毒症诱导的急性肾损伤(AKI)的影响，并探索其可能的作用机制。方法 10~12周龄健康雄性Balb/c小鼠30只随机分为假手术组（sham组，n=10），盲肠结扎穿孔组（CLP组，n=10），rPCT免疫组（免疫组，n=10）。使用盲肠结扎穿孔法（CLP）制作小鼠脓毒症动物模型。于CLP术前3周、2周、1周使用rPCT 50 μg腹腔内注射免疫小鼠。CLP术后24 h检测小鼠血清肌酐(SCr)、尿素氮(BUN)、降钙素原(PCT)、C-反应蛋白（CRP）、促炎介质和抗炎介质水平。处死小鼠，苏木精-伊红（HE）染色，观察小鼠肾组织病理学变化。〖HTH〗结果〓〖HTSS〗与假手术组比较，CLP组小鼠SCr、BUN、TNF-α、IL-1及IL-6明显升高（P＜0.01）。与CLP组比较，免疫组小鼠SCr、BUN、TNF-α、IL-1及IL-6明显下降（P＜0.05）。假手术组小鼠肾脏组织病理学基本正常；CLP组小鼠肾脏组织病理学显示，肾小管上皮细胞明显肿胀、脱落、空泡变性、刷状缘明显破坏，肾间质中性粒细胞浸润；免疫组小鼠肾小管上皮细胞肿胀、变性减轻。Kaplan-Meier生存分析显示，免疫组小鼠7 d病死率较CLP组低（P＜0.05）。〖HTH〗结论〓〖HTSS〗PCT在脓毒症相关性AKI的发病过程中可能促进炎症介质的释放，从而加重病情的进展。rPCT预先免疫小鼠，可以在一定程度上对脓毒症相关性AKI起保护作用。

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	李虎
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	黄力鸥
	唐建国

收稿日期: 2017-08-01

基金资助:上海市闵行区卫生和计划生育委员会科研课题（2014MW03）

通讯作者: 唐建国（1968-），男，硕士，主任医师，教授，硕士生导师，GICU主任，E-mail:tangjianguo@5thhospital.com。

作者简介: 李虎（1984-），男，硕士，主治医师，E-mail: lihu32782702@163.com。

引用本文:

李虎，杨春辉，崔宇慧，黄力鸥，唐建国. 重组降钙素原免疫小鼠对脓毒症诱导的急性肾损伤的保护作用[J]. 中国急救医学, 2018, 38(2): 129-132.
Li Hu, Yang Chun-hui, Cui Yu-hui, Huang Li-ou, Tang Jian-guo. Protective effects of immunization with recombinant procalcitonin on sepsis-induced acute kidney injury in mouse. Chinese Journal of Critical Care Medicine, 2018, 38(2): 129-132.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.02.008 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I2/129

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