创伤性脑损伤后小鼠海马区N-甲基-D-天冬氨酸受体NR2B亚基的表达变化和意义

doi:10.3969/j.issn.1002-1949.2019.10.016

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摘要目的探讨创伤性脑损伤（TBI）后小鼠海马区N-甲基-D-天冬氨酸（NMDA）受体2B亚基(NR2B)及其磷酸化(pNR2B)的表达变化和意义。方法健康雄性Balb/c小鼠72只，随机均分为三组：假损伤组(sham组)、脑损伤组(TBI组)、Ifenprodil治疗干预组(TBI+IF组)。TBI组小鼠予以控制性皮层损伤打击，建立TBI模型。TBI+IF组小鼠在TBI后予以Ifenprodil腹腔注射1周。伤后第1、3、7天行Western-blotting检测，分析各组小鼠海马区NR2B和pNR2B的表达水平。伤后7~11 d行Morris水迷宫试验，分析各组小鼠的认知功能。结果 TBI组海马区NR2B和pNR2B表达水平在伤后1 d显著增加，分别为3.54±0.15和3.27±0.36，伤后3 d、7 d逐渐降低至1.21±0.11和1.28±0.07，但仍高于sham组表达水平(0.47±0.03和0.39±0.04，P<0.05)。与TBI组比较，各时点TBI+IF组海马区NR2B和pNR2B表达水平均显著降低，在伤后1 d表达水平(2.08±0.32和2.15±0.27) 差异最显著。水迷宫试验结果显示，TBI组逃避潜伏期(29.39±4.67)s较sham组(17.55±2.90)s显著增加（P<0.05），穿越站台的次数和原站台象限停留时间［2.97±0.63和（19.57±4.08）s］较sham组［12.01±3.65和(48.67±6.13)s］显著减少(P<0.05)。与TBI组比较，TBI+IF组逃避潜伏期(15.89±2.90)s显著减少(P<0.05)，穿越站台的次数和原站台象限停留时间［10.41±2.54和（51.82±9.26)s］显著增加(P<0.05)。结论 TBI后小鼠海马区NMDA受体的NR2B亚基表达及磷酸化水平增加，抑制NR2B可改善TBI后认知功能障碍。

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	王玮
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关键词 ：创伤性脑损伤（TBI）, N-甲基-D-天冬氨酸（NMDA）, NR2B, 认知功能

基金资助:国家自然科学基金面上项目(81871587)；国家自然科学基金青年项目(81601671)

通讯作者: 尹文（1965-），男，硕士，主任医师，E-mail:xjyyyw@126.com。

作者简介: 王玮（1982-），男，硕士，主治医师，E-mail:willisw@163.com。

引用本文:

王玮，王倩梅，尹文. 创伤性脑损伤后小鼠海马区N-甲基-D-天冬氨酸受体NR2B亚基的表达变化和意义[J]. 中国急救医学, 2019, 39(10): 998-1002.
Wang Wei, Wang Qian-mei, Yin Wen. Expression and significance of hippocampal NMDA receptor subunit NR2B after traumatic brain injury in mice. Chinese Journal of Critical Care Medicine, 2019, 39(10): 998-1002.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2019.10.016 或 http://www.cnemergency.org.cn/CN/Y2019/V39/I10/998

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