保留与切除椎板制备小鼠急性脊髓损伤模型的比较研究

doi:10.3969/j.issn.1002-1949.2016.05.0016

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摘要目的比较应用自制的脊髓打击仪制备保留与切除椎板小鼠急性脊髓损伤（SCI）模型的优缺点，探讨进一步改进小鼠急性脊髓损伤模型的建立方法。方法 6 周龄SPF 级雌性KM小鼠30 只，随机分成三组（每组10 只）。A 组为假手术组；B 组为切除椎板40 g 打击组；C 组为保留椎板40 g 打击组。分别记录手术操作时间、出血量、切口长度，且于术后1～8 周的不同时间点行BMS 评分、足迹分析及组织学检查，评价小鼠急性脊髓损伤的程度。结果保留椎板组手术时间（36.4±5.21）min、切口长度（1.32±0.08）cm、出血量（0.14±0.03）mL；椎板切除组手术时间（45.7±5.48）min、切口长度（1.7±0.08）cm、出血量（0.24±0.05）mL，保留椎板组均优于切除椎板组（P＜0.05）；两组Basso 小鼠后肢行为学（BMS）评分、足迹分析、组织学检查均相似。结论保留椎板的小鼠急性脊髓损伤模型与切除椎板一样能有效显示脊髓损伤的行为学及病理学过程，且操作较切除椎板模型更加简单、快捷，手术创伤更小，更有利于后续科学研究的开展。

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	陈学周
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	尤涛

收稿日期: 2015-12-18

基金资助:国家自然科学基金资助项目（81171797）；湖北省自然科学基金资助项目（2011CHB022）；中国博士后科学基金（9021346201）；安徽省博士后科学基金（9021346202）

通讯作者: 尤涛（1974-），男，博士，副主任医师，副教授，E-mail：youtaowh@163.com

作者简介: 陈学周（1991-），男，硕士研究生。

引用本文:

陈学周，毕意辉，张可可，董洲，张明凯，尤涛. 保留与切除椎板制备小鼠急性脊髓损伤模型的比较研究[J]. 中国急救医学, 2016, 36(5): 451-454.
CHEN Xue-zhou, BI Yi-hui, ZHANG Ke-ke, DONG Zhou, ZHANG Ming-kai, YOU Tao. Comparison on two kinds of spinal cord injury model in mice with self-made spinal impactor device. Chinese Journal of Critical Care Medicine, 2016, 36(5): 451-454.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2016.05.0016 或 http://www.cnemergency.org.cn/CN/Y2016/V36/I5/451

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