外源性硫化氢对小鼠动脉粥样硬化的调节作用及机制

doi:10.3969/j.issn.1002-1949.2018.01.021

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摘要目的：研究外源性硫化氢（H2S）通过Toll样受体4（TLR4）对ApoE-/-小鼠动脉粥样硬化的调控作用及机制。方法：制作ApoE-/-小鼠颈动脉粥样硬化不稳定斑块模型，外源性H2S缓释剂GYY4137干预。模型分为正常对照组、不稳定斑块组、不稳定斑块组+不同剂量GYY4137组、不稳定斑块+LY294002组。检测小鼠血脂水平，油红染色检测粥样硬化斑块，ELISA法检测血浆炎症因子TNF-α、IL-6、IL-1和IL-10，Western blot检测斑块组织内TLR4、PI3K、Akt、磷酸化Akt（P-Akt）蛋白表达。结果：与对照组比较，模型组脂质成分及促炎因子TNF-α、IL-6、IL-1增加，抑炎因子IL-10减少，粥样斑块内TLR4、PI3K、P-Akt蛋白表达增加（P＜0.01）。GYY4137中、高剂量干预减少模型组脂质成分及促炎因子TNF-α、IL-6、IL-1表达，增加抑炎因子IL-10，减少TLR4、PI3K、P-Akt蛋白表达（P＜0.01）。LY294002干预效果与GYY4137类似。结论：外源性H2S能调控TLR4及TNF-α、IL-6、IL-1、IL-10表达，防止ApoE-/-小鼠颈动脉粥样斑块发生、发展，作用与PI3K-Akt信号通路有关。

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	杨琴
	姜红
	文渊
	何隆淼
	张明霞
	黄俊

收稿日期: 2017-09-01

基金资助:江西省科技厅科技计划项目（20151BBG70222）

通讯作者: 黄俊（1968-），男，博士，教授，硕士生导师，E-mail:junhuang918@163.com。

作者简介: 杨琴（1986-），女，硕士，E-mail：yangqi007@126.com。

引用本文:

杨琴，姜红，文渊，何隆淼，张明霞，黄俊. 外源性硫化氢对小鼠动脉粥样硬化的调节作用及机制[J]. 中国急救医学, 2018, 38(1): 94-96.
Yang Qin, Jiang Hong, Wen Yuan, He Long-miao, Zhang Ming-xia, Huang Jun. The effect and mechanism of exogenous hydrogen sulfide on atherosclerosis in apolipoprotein E-/-mice. Chinese Journal of Critical Care Medicine, 2018, 38(1): 94-96.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.01.021 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I1/94

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