中性粒细胞来源微颗粒引起大鼠急性呼吸窘迫综合征的病理学变化

doi:10.3969/j.issn.1002-1949.2018.01.020

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摘要目的：探讨中性粒细胞来源微颗粒（NDMPs）对大鼠急性呼吸窘迫综合征（AROS）肺组织病理学变化的影响。方法：采集大鼠外周静脉血后提取中性粒细胞，并用脂多糖（LPS）对中性粒细胞进行刺激后提取 NDMPs，用纳米颗粒跟踪分析仪对其进行定性、定量后，采用随机分组的方法将实验大鼠分为三组：正常对照组、LPS 组和NDMPs 组（每组n=10），经支气管内分别滴入生理盐水100 μL、LPS (5 mg/kg)、NDMPs (2.0×109/kg)，24 h后取材苏木精-伊红（HE）染色，观察肺组织学变化、测肺湿干质量比、肺组织髓过氧化物酶(MPO)活性及支气管肺泡灌洗液中各种炎性因子的表达。结果：与正常对照组比较，LPS 组和NDMPs 组的肺组织HE染色表现出大量炎症浸润、肺泡及间质出血、水肿等病理学变化；LPS 组和NDMPs 组的肺损伤评分及肺湿干质量比高于对照组（P＜0.05），NDMPs组的肺损伤评分低于LPS 组（P＜0.01），NDMPs 组的肺湿干质量比低于LPS 组（P＜0.05）；肺组织MPO活性及支气管肺泡灌洗液（BALF）中炎性因子TNF-α和IL-10的表达均高于正常对照组（P＜0.01），LPS组的支气管肺泡灌洗液中炎性因子IL-1β的表达高于正常对照组（P＜0.01），NDMPs 组的支气管肺泡灌洗液中炎性因子IL-1β的表达高于正常对照组（P＜0.05），NDMPs组的支气管肺泡灌洗液中炎性因子TNF-α、IL-1β和IL-10的表达均低于LPS组（P＜0.05）。结论： LPS诱导产生的NDMPs可引起大鼠ARDS的病理学变化。

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	王向蒙
	肖文凯
	邓烈华

收稿日期: 2017-08-02

基金资助:广东省自然科学基金（S201301001528）

通讯作者: 邓烈华（1972-），男，博士，主任医师，硕士研究生导师，ICU主任，E-mail:glinson@126.com。

作者简介: 王向蒙（1989-），女，硕士，住院医师，E-mail：missmeng1106@126.com。

引用本文:

王向蒙，肖文凯，邓烈华. 中性粒细胞来源微颗粒引起大鼠急性呼吸窘迫综合征的病理学变化[J]. 中国急救医学, 2018, 38(1): 89-93.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.01.020 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I1/89

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