瘦素对新生期大鼠惊厥性脑损伤的保护作用及对海马〖JP〗CB-D28k表达的影响

doi:10.3969/j.issn.1002-1949.2019.03.016

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摘要目的探讨瘦素急性期干预对新生期大鼠惊厥性脑损伤的神经保护作用及海马组织CB-D28k表达的影响。方法采用随机数字表法将 7日龄SD大鼠分为四组，即单纯对照组、瘦素对照组（Leptin组）、单纯惊厥组（RS组）及瘦素干预组（RS+Leptin组）。RS组和RS+Leptin组大鼠应用三氟乙醚诱导惊厥持续状态，连续7 d。Leptin组和RS+Leptin组于每日惊厥后1 h腹腔给予瘦素4 mg/kg,连续干预7 d（P7～P13）。于P14天，即末次惊厥后24 h，每组随机取5只，取海马组织，采用蛋白免疫印迹技术（Western blot）检测海马CB-D28k的表达，采用实时PCR技术检测海马CB-D28k mRNA的表达。每组余10只于P33进行避暗测试。结果四组大鼠错误次数和潜伏期组间差异有统计学意义（F=5.471，F=13.775，P<0.05），RS组大鼠较对照组错误次数明显增多（次：1.40±0.52 vs. 0.80±0.42，P<0.05），RS+Leptin组大鼠较RS组错误次数明显减少(次：0.60±0.52 vs.1.40±0.52，P<0.05)；RS组大鼠较对照组潜伏期明显缩短（s：92.01±22.78 vs. 154.97±23.65，P<0.05），RS+Leptin组大鼠较RS组潜伏期明显延长（s:142.42±21.85 vs.92.01±22.78, P<0.05）。CB-D28k及mRNA在四组大鼠海马组织中表达差异有统计学意义（F=26.963，F=24.420，P<0.05）；与对照组比较，RS组海马组织CB-D28k及mRNA的表达量明显减少（1.29±0.20 vs.0.44±0.09，1.08±0.16 vs.0.49±0.10，P<0.05）；与RS组比较，RS+Leptin组海马组织CB-D28k及mRNA的表达量明显增加（0.44±0.09 vs.1.22±0.21，0.49±0.10 vs. 0.93±0.09，P<0.05）。结论急性期瘦素干预对新生期大鼠惊厥所致神经行为损伤具有神经保护作用，其作用机制可能与调节海马组织CB-D28k表达有关。

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	李亚超
	李丽丽
	金梅芳
	高媛媛
	陈妮娜
	王丹丹
	倪宏

关键词 ：瘦素, 惊厥, CB-D28k

基金资助:国家自然科学基金(81471337，81271458）；江苏省普通高校研究生科研创新项目（KYCX18-2537）

通讯作者: 倪宏（1968-），男，博士生导师，教授，E-mail:nhdoctor@163.com。

作者简介: 李亚超（1991-），女，硕士研究生，E-mail:szdxlyc@126.com。

引用本文:

李亚超，李丽丽，金梅芳，高媛媛，陈妮娜，王丹丹，倪宏. 瘦素对新生期大鼠惊厥性脑损伤的保护作用及对海马〖JP〗CB-D28k表达的影响[J]. 中国急救医学, 2019, 39(3): 280-284.
Li Ya-chao, Li Li-li, Jin Mei-fang, Gao Yuan-yuan, Chen ni-na, Wang Dan-dan, Ni Hong. Protective effect of leptin on seizure brain injury in neonatal rats and its effect on expression of CB-D28k in hippocampus. Chinese Journal of Critical Care Medicine, 2019, 39(3): 280-284.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2019.03.016 或 http://www.cnemergency.org.cn/CN/Y2019/V39/I3/280

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