外源性硫化氢对脓毒症巨噬细胞氧化还原平衡的影响

doi:10.3969/j.issn.1002-1949.2019.01.016

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摘要目的研究外源性硫化氢对脓毒症巨噬细胞氧化还原平衡的影响。方法培养小鼠腹腔巨噬细胞株J774，分为缓冲液（PBS）（1 mL/mL）对照组、脂多糖（LPS）（1 μg/mL）组、炔丙基甘氨酸（PAG）（1 mmol/L）+LPS（1 μg/mL）组、硫化氢缓释供体（GYY4137）（500 μmol/L）组、GYY4137（500 μmol/L）+LPS（1 μg/mL）组，酶联免疫吸附（ELISA）法测定各组细胞培养上清中活性氧（ROS）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px），电子显微镜观察细胞内线粒体形态的变化。结果最终测得LPS组细胞上清中ROS为（90.293±0.217）U/mL，MDA为（1.665±0.068）nmol/mL，SOD为（89.857±3.00）U/mL，CAT为（8.099±0.130）U/mL，GSH-Px为（10.287±0.335）μmol/L。〖JP2〗GYY4137+LPS组细胞上清中ROS平均浓度为（25.8192.988）U/mL，MDA为（0.355±0.065）nmol/mL，SOD为（189.690±0.290）U/mL，CAT为（189.690±0.290）U/mL，GSH-Px为（287.235±0.864）μmol/L。与LPS组比较，GYY4137+LPS组氧化应激产物ROS及氧化应激标志物MDA水平较低（P<0.05），抗氧化剂SOD、CAT、GSH-Px水平较高（P<0.05），细胞及线粒体损伤程度也较小。结论硫化氢对脓毒症巨噬细胞氧化还原平衡有一定的保护作用，这种保护作用的机制可能是硫化氢减轻了脓毒症时线粒体的氧化应激损伤。

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	王兴程
	杨磊
	王斌生
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	马立斌
	柴琛

关键词 ：硫化氢, 脓毒症, 巨噬细胞, 氧化应激

基金资助:兰州市科技局项目（2015-2-65）

通讯作者: 柴琛（1974-），男，教授，硕士生导师，主任医师，E-mail: chasechai@126.com。

作者简介: 王兴程（1993-），男，硕士研究生，E-mail: 1499048708@qq.com。

引用本文:

王兴程，杨磊，王斌生，彭超，马立斌，柴琛. 外源性硫化氢对脓毒症巨噬细胞氧化还原平衡的影响[J]. 中国急救医学, 2019, 39(1): 77-81.
Wang Xing-cheng, Yang Lei, Wang Bin-sheng, Peng Chao, Ma Li-bin, Chai Chen. Effects of exogenous hydrogen sulfide on redox equilibrium of septic macrophages. Chinese Journal of Critical Care Medicine, 2019, 39(1): 77-81.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2019.01.016 或 http://www.cnemergency.org.cn/CN/Y2019/V39/I1/77

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