生长激素释放肽对失血性休克大鼠心肌保护作用及对核因子-κB的影响

doi:10.3969/j.issn.1002-1949.2019.01.014

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摘要目的观察生长激素释放肽（ghrelin）对失血性休克（hemorrhagic shock, HS）大鼠心肌的保护作用及对核因子-κB （NF-κB）表达的影响。方法 32只成年雄性SD大鼠随机分为四组：假手术组（sham）、假手术+ghrelin组（sham+ghrelin）、HS组、HS+ghrelin组，每组8只。经大鼠股动脉放血使平均动脉压（MAP）维持在40 mm Hg左右1 h，然后静脉给予10 nmol/kg的ghrelin或等量生理盐水注射，随后立即用大鼠自体血和等量乳酸林格液复苏并观察2 h，同时进行血流动力学监测。复苏结束后，采集心肌组织行病理学检查；检测心肌丙二醛(MDA)、超氧化物歧化酶(SOD)活性及髓过氧化物酶（MPO）活性；取大鼠血浆测定肿瘤坏死因子-α(TNF-α)和白细胞介素-6 (IL-6)水平；取心肌组织测定NF-κB蛋白的表达。结果在复苏后相同时间点，HS组大鼠MAP较sham组显著降低（P<0.05），ghrelin处理后能够显著缓解HS大鼠复苏后的MAP降低程度（P<0.05）；与sham组比较，HS组大鼠心肌损伤程度、MDA含量、MPO活性、血浆炎症因子表达以及NF-κB蛋白表达显著增高，SOD活性显著降低（P<0.05）；与HS组比较，HS+ghrelin组心肌损伤程度、MDA含量、MPO活性、血浆炎症因子表达以及NF-κB蛋白表达显著降低［MDA：（2.86±0.08）nmol/mg prot vs.(1.45±0.29)nmol/mg prot，MPO：（2.23±0.20）U/g prot vs. (1.87±0.22)U/g prot, TNF-α:(62.71±4.31)pg/mL vs. (35.16±4.06)pg/mL, IL-6:(73.33±13.94)pg/mL vs.(42.22±7.92)pg/mL, P<0.05］， SOD活性显著增强［(72.77±4.90)U/mg prot vs. (89.31±7.35)U/mg prot, P<0.05］。结论 Ghrelin对HS大鼠心肌有保护作用，这种保护机制与抑制NF-κB信号通路的表达有关。

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	张丽娜
	岳子勇

关键词 ：失血性休克（HS）, 心肌保护, 生长激素释放肽（ghrelin）, 核转录因子-κB(NF-κB)

基金资助:国家自然科学基金项目(30772085)

通讯作者: 岳子勇（1968-），男，博士后，主任医师，硕士生导师， E-mail：yueziyong@126.com。

作者简介: 张丽娜（1987-），女，硕士，住院医师，E-mail：826790447@qq.com。

引用本文:

张丽娜，岳子勇. 生长激素释放肽对失血性休克大鼠心肌保护作用及对核因子-κB的影响[J]. 中国急救医学, 2019, 39(1): 66-70.
Zhang Li-na, Yue Zi-yong. Effect of exogenous ghrelin on myocardial protection and NF-κB in rats after hemorrhagic shock. Chinese Journal of Critical Care Medicine, 2019, 39(1): 66-70.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2019.01.014 或 http://www.cnemergency.org.cn/CN/Y2019/V39/I1/66

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