胍丁胺对大鼠高氧性急性肺损伤的影响

doi:10.3969/j.issn.1002-1949.2019.09.016

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摘要目的探讨胍丁胺对大鼠高氧性急性肺损伤(HALI)的影响，为HALI药物治疗寻找理论依据。方法清洁级SD大鼠48只，随机分为6组，每组8只（n=8），即对照组、高氧组、胍丁胺A组（胍丁胺100 mg/kg）、胍丁胺B组（胍丁胺200 mg/kg）、胍丁胺C组（胍丁胺400 mg/kg）和胍丁胺D组（胍丁胺800 mg/kg）。对照组大鼠空气中饲养，高氧组、胍丁胺A组、胍丁胺B组、胍丁胺C组和胍丁胺D组大鼠高氧箱中饲养，分别在建模48 h抽取大鼠动脉血行血气分析，计算氧合指数（OI）和呼吸指数(RI)；取大鼠左下肺测量大鼠肺湿/干质量（W/D）；检测肺泡灌洗液(BALF)中TNF-α、 IL-6、IL-1含量及SOD、MDA的活性。结果 ①与对照组比较，高氧组及胍丁胺各组大鼠OI、SOD明显降低（均P＜0.05），W/D、RI、TNF-α、IL-6、IL-1明显升高（均P＜0.05）；与高氧组比较，胍丁胺各组大鼠OI、SOD明显增高（均P＜0.05）；W/D、RI、TNF-α、IL-6、IL-1明显降低（均P＜0.05）。②胍丁胺A组、B组、C组之间比较，随着胍丁胺计量增加，各组大鼠OI逐渐增高，RI逐渐降低，而胍丁胺C组与胍丁胺D组大鼠之间比较差异无统计学意义（P>0.05）。③对照组大鼠肺组织肺泡结构正常；高氧组及胍丁胺各组大鼠肺组织结构明显紊乱，肺泡壁断裂、破坏及融合成肺大泡，肺泡间隔明显水肿、增宽，大量炎性细胞浸润，肺泡腔内出现水肿液及炎性细胞，可见少量红细胞；但随着胍丁胺计量增加，胍丁胺B组、胍丁胺C组及胍丁胺D组大鼠肺泡结构破坏逐渐减轻，而胍丁胺C组与胍丁胺D组大鼠肺泡结构变化不大。结论胍丁胺对大鼠HALI具有明确的保护作用，且最佳保护计量可能是400 mg/kg。

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关键词 ：胍丁胺, 高氧性急性肺损伤(HALI), 氧合指数（OI), 呼吸指数(RI), 病理, 肺泡灌洗液(BALF)

基金资助:国家自然科学基金（81560019）；贵州省科技计划项目（黔科合LH字［2017］7115）

通讯作者: 陈淼（1964-），女，硕士，主任医师，E-mail:764590955@qq.com。

作者简介: 刘国跃（1986-），男，硕士，主治医师，E-mail：376051838@qq.com。

引用本文:

刘国跃，王勇，陈淼. 胍丁胺对大鼠高氧性急性肺损伤的影响[J]. 中国急救医学, 2019, 39(9): 882-886.
Liu Guo-yue, Wang Yong, Chen Miao. Effects of agmatine on hyperoxia-induced acute lung injury in rats〓〖WTBX〗〖STBX〗Liu Guo-yue, Wang Yong, Chen Miao. Chinese Journal of Critical Care Medicine, 2019, 39(9): 882-886.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2019.09.016 或 http://www.cnemergency.org.cn/CN/Y2019/V39/I9/882

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