Morris水迷宫联合神经功能评分法构建脓毒症脑病大鼠模型的研究

doi:10.3969/j.issn.1002-1949.2018.06.013

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摘要目的在脑电生物监测方法构建脓毒症脑病动物模型的基础上，进一步探讨和改良脓毒症脑病大鼠模型。方法 80只SD大鼠放置脑电监测电极后采用经典盲肠结扎穿孔(cecal ligation and puncture ，CLP)方法诱发脓毒症，术后分别根据脑电图(electroencephalogram，EEG)监测法(简称EEG法)和Morris水迷宫(Morris water maze, MWM)实验联合神经功能评分法(简称MWM法)将大鼠分为脓毒症脑病(sepsis associated encephalopathy, SAE)组和非脓毒症脑病(non-sepsis associated encephalopathy，NSAE)组，比较两种诊断方法的差异。结果两种诊断方法具有较高一致性，且与后期标本病理改变相符。结论 SAE大鼠EEG方法模型稳定性欠佳，而MWM法鉴别脓毒症脑病与EEG法具有较好的一致性，可以用于脓毒症脑病动物模型筛查。

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	陈采霞
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	王运佳
	艾宇航

关键词 ：脓毒症脑病（SAE）, 动物模型, Morris水迷宫（MWM）, 神经功能评分

基金资助:国家自然科学基金青年科学基金(81401099)

通讯作者: 艾宇航（1961-），女，博士，主任医师，E-mail:ayhicu1978@126.com。

作者简介: 陈采霞（1990-），女，硕士研究生，E-mail: chencheese@qq.com。

引用本文:

陈采霞，张丽娜，吴龙，邓松筠，王运佳，艾宇航. Morris水迷宫联合神经功能评分法构建脓毒症脑病大鼠模型的研究[J]. 中国急救医学, 2018, 38(6): 518-.
Chen Cai-xia, Zhang Li-na, Wu Long, Deng Song-yun, Wang Yun-jia, Ai Yu-hang. Morris water maze joint nerve function score method to build sepsis associated encephalopathy rat model. Chinese Journal of Critical Care Medicine, 2018, 38(6): 518-.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.06.013 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I6/518

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