microRNA-222通过Akt信号通路调控人外周血内皮祖细胞迁移

doi:10.3969/j.issn.1002-1949.2018.03.007

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摘要目的探讨microRNA-222(miR-222)通过调控Akt信号通路对内皮祖细胞(EPCs)迁移功能的影响及对靶向调控转录因子ETS-1(ETS proto-oncogene 1, transcription factor)的影响。方法从正常成人外周静脉血分离、体外培养后鉴定EPCs。分别向人内皮祖细胞(hEPCs)转染人工合成的miR-222的模拟物(mimic)、抑制物(inhibitor)和相应的阴性对照(NC)。用荧光定量PCR法测miR-222及ETS-1 mRNA的表达。应用Western blot测量每组的ETS-1、p-Akt和Akt的蛋白表达。各组EPCs用Transwell检测其迁移。结果 miR-222可以靶向影响ETS-1的mRNA和蛋白表达。与对照组比较，上调miR-222表达可致ETS-1的mRNA和蛋白表达降低，同时抑制EPCs的迁移能力(均P＜0.05)。相反，下调miR-222表达可致ETS-1的mRNA和蛋白表达升高，同时增强EPCs的迁移能力(均P＜0.05)。Western blot检测显示，上调miR-222表达抑制p-Akt的蛋白水平；下调miR-222表达增强p-Akt的蛋白水平(均P＜0.05)。结论 miR-222可通过调控Akt信号通路和靶向转录因子ETS-1影响EPCs的迁移功能。

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	许文峰
	王翠平
	张浩
	蔡华忠
	施良
	孙文文
	任国庆

关键词 ：内皮祖细胞（EPCs）, microRNA-222, 转录因子（miR-222）, Akt信号通路

基金资助:国家自然科学基金青年项目（81400269）；江苏省青年医学重点人才（QNRC2016837）；镇江市社会发展科技支撑项目（SH2016039）；江苏大学临床专项基金（JDLCZX012）

通讯作者: 任国庆（1964-），男，硕士，主任医师，硕士生导师，急诊科主任，E-mail: doctorgq@sohu.com。

作者简介: 许文峰（1991-），男，硕士，住院医师。

引用本文:

许文峰，王翠平，张浩，蔡华忠，施良，孙文文，任国庆. microRNA-222通过Akt信号通路调控人外周血内皮祖细胞迁移 [J]. 中国急救医学, 2018, 38(3): 208-212.
Xu Wen-feng, Wang Cui-ping, Zhang Hao, Cai Hua-zhong, Shi Liang, Sun Wen-wen, Ren Guo-qing. MicroRNA-222 modulates the migratory ability of endothelial progenitor cells from human peripheral blood through the Akt signaling pathway . Chinese Journal of Critical Care Medicine, 2018, 38(3): 208-212.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.03.007 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I3/208

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