高良姜素对LPS诱导THP-1细胞炎症的保护作用

doi:10.3969/j.issn.1002-1949.2016.04.017

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摘要目的探讨高良姜素对脂多糖（LPS）诱导人急性单核细胞白血病细胞株 THP-1 细胞炎症的保护作用及其可能的机制。方法 MTS 法检测细胞活性；酶联免疫吸附（ELISA）法检测TNF-α、MCP-1、IL-1β、IL-6 的分泌；免疫印迹（Western blot）法检测 NF-κB 以及 MAPK 通路相关蛋白的变化。结果高良姜素在 0~20 μmol/L 范围内对细胞活力无明显影响。LPS 的用药浓度为500 ng/mL；与 LPS 模型组相比，高良姜素 2.5~20 μmol/L 能有效抑制炎症因子 TNF-α、MCP-1、IL-1β、IL-6 的释放（P＜0.05）；高良姜素 20 μmol/L 能抑制 NF-κB 通路 IκBα的磷酸化，从而减少核转录因子（NF）-κB 的核转位，但对 MAPK 通路无影响。结论高良姜素对 LPS 刺激的 THP-1 细胞炎症具有一定的保护作用，其保护机制可能与其调控 NF-κB 通路，抑制 IκBα的磷酸化，减少 NF-κB的核转录，进而抑制 TNF-α、MCP-1 、IL-1β、IL-6 的表达有关。

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	孔田玉
	张振辉
	胡欢
	辜洪娇
	熊旭明

收稿日期: 2015-12-25

基金资助:广东省科技厅项目（2012B031800233）

通讯作者: 熊旭明（1963-），男，教授，主任医师，硕士生导师，E-mail: xiongxuming9@126.com。

作者简介: 孔田玉（1990-），女，硕士研究生，E-mail: ktyfancy@126.com。

引用本文:

孔田玉，张振辉，胡欢，辜洪娇，熊旭明. 高良姜素对LPS诱导THP-1细胞炎症的保护作用[J]. 中国急救医学, 2016, 36(4): 362-366.
KONG Tian-yu，ZHANG Zhen-hui,HU-Huan,GU Hong-jiao,XIONG Xu-ming. . Protective effect of galangin on the LPS-induced inflammation in THP-1 cells. Chinese Journal of Critical Care Medicine, 2016, 36(4): 362-366.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2016.04.017 或 http://www.cnemergency.org.cn/CN/Y2016/V36/I4/362

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