不同剂量6-姜酚对大鼠心肌缺血-再灌注损伤的保护作用

doi:10.3969/j.issn.1002-1949.2018.11.014

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摘要目的探讨不同剂量6-姜酚（6-G）对大鼠心肌缺血-再灌注损伤（MIRI）的保护作用。方法将大鼠冠状动脉左前降支（LAD）结扎0.5 h制备大鼠MIRI模型。40只Sprague-Dawley（SD）大鼠随机分为5组：假手术组（Sham组）、MIRI对照组（I/R组）、6-G低、中、高剂量组，每组8只。Sham组仅开胸，不行LAD结扎；I/R组制作MIRI模型；6-G各组手术前0.5 h经尾静脉给不同剂量6-G（3、6、12 mg/kg）。各组大鼠经酶联免疫吸附法（ELISA法)检测血清白细胞介素(IL)-6、血清肿瘤坏死因子（TNF）-α、血清磷酸肌酸激酶(CK)、血清磷酸肌酸激酶-同工酶（CK-MB）和血清肌钙蛋白T(cTnT)水平。使用生物机能实验系统（BL-420S）采集射血分数（LVEF）、左心室缩短分数（LVFS）、左心室收缩压（LVSP）、左心室舒张末期压力（LVEDP）、等容收缩期左心室内压力上升/下降的最大速率(±dp/dtmax)。苏木精-伊红（HE）染色，光镜下观察心肌病理学变化。结果与Sham组比较，其余各组IL-6、TNF-α水平升高(P<0.05)；心肌酶（CK、CK-MB）和cTnT水平升高(P<0.05)；心功能指标LVEF、LVFS、LVSP、+dp/dtmax、-dp/dtmax降低(P<0.05)，而LVEDP升高(P<0.05)。与I/R组比较，中剂量组IL-6［（34.80±5.10）pg/mL vs.（46.95±5.10）pg/mL］、TNF-α［（30.39±3.93）pg/mL vs.（40.46±3.39）pg/mL］水平降低(P<0.05)；CK［（1.35±0.02）mU/mL vs. （1.48±0.03）mU/mL］、CK-〖JP2〗MB［（1.980.41）ng/mL vs.（2.59±0.60）ng/mL］和cTnT［（117.00±13.67）pg/mL vs. （148.55±14.76）pg/mL］水平下降(P<0.05)；LVEF［（41.38±3.74）% vs.（30.00±4.69）%］、LVFS［（34.63±4.44）% vs.（21.25±4.43）%］、LVSP［（137.13±5.41）mm Hg vs.（124.75±4.17）mm Hg］、+dp/dtmax［（4323.25±398.17）mm Hg/s vs. （3530.36±245.02）mm Hg/s］、-dp/dtmax［（3188.38±248.06）mm Hg/s vs. （2395.25±315.60）mm Hg/s］升高(P<0.05)，而LVEDP［（2.310.58）mm Hg vs.（3.65±0.44）mm Hg］降低(P<0.05)；心肌组织损伤减轻。而低、高剂量组IL-6、TNF-α、心肌酶和cTnT、心功能指标变化差异无统计学意义（P>0.05）；心肌组织损伤未见显著减轻。结论中剂量6-姜酚(6 mg/kg)可减轻MIRI，其机制可能通过抑制炎症反应，保护心肌细胞，改善大鼠心脏功能。

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	赵位昆
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关键词 ： 6-姜酚(6-G), 心肌缺血-再灌注损伤（MIRI）, 炎症反应, 肿瘤坏死因子-α(TNF-α), 白细胞介素-6 (IL-6)

基金资助:国家自然科学基金资助项目(81760861)；桂林市科学研究与技术开发计划项目（20160226-1-1）；广西研究生教育创新计划项目（YCBZ2017034）

通讯作者: 徐彤彤（1968-），硕士，主任医师，教授，硕士生导师，科主任，E-mail：2655820200@qq.com。

作者简介: 赵位昆（1990-），男，硕士研究生，E-mail：120806119@qq.com。

引用本文:

赵位昆，徐彤彤，吕祥威，武琦，覃秋语. 不同剂量6-姜酚对大鼠心肌缺血-再灌注损伤的保护作用[J]. 中国急救医学, 2018, 38(11): 991-995.
Zhao Wei-kun, Xu Tong-tong, Lu Xiang-wei, Wu Qi, Qin Qiu-yu. Protective effects of different doses of 6-Gingerol on myocardial ischemia-reperfusion injury in rats. Chinese Journal of Critical Care Medicine, 2018, 38(11): 991-995.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.11.014 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I11/991

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