姜黄素预处理对干热环境下中暑大鼠肠黏膜相关蛋白及血清炎症因子的影响

doi:10.3969/j.issn.1002-1949.2018.10.015

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摘要目的探讨姜黄素预处理对干热下中暑大鼠肠黏膜热休克蛋白70（HSP70）、高迁移率族蛋白1（HMGB1）、细胞间黏附分子-1（ICAM-1）表达及血清炎症因子的影响。方法 30只SD大鼠随机分为正常对照组（NC组）、干热中暑组（DH组）及姜黄素灌胃组（Cur组），每组各10只。NC组、DH组生理盐水灌胃，Cur组按200 mg/kg姜黄素灌胃（连续7 d，每天1次）。DH组和Cur组灌胃结束后将其置于模拟干热环境的实验舱中，温度为（41±0.5）℃，相对湿度为（10±1）%，干热暴露150 min造成重度中暑模型后，Western blotting检测回肠组织中HSP70、HMGB1和ICAM-1表达，ELISA检测血液中内毒素（LPS）和炎症因子TNF-α、IL-1β及IL-6的水平。结果 DH组HSP70、HMGB1和ICAM-1表达及LPS、TNF-α、IL-1β、IL-6浓度较NC组显著增加（P<0.05）；Cur组HSP70表达（8.75±0.46）较DH组（2.17±0.14）显著增加（P<0.05），Cur组HMGB1、ICAM-1表达（6.95±0.42、9.31±0.76）较DH组（17.76±1.54、19.39±1.64）显著降低（P<0.05），Cur组LPS、TNF-α、IL-1β、IL-6浓度较DH组显著降低（P<0.05）。结论姜黄素预处理可能通过调控肠黏膜HSP70、HMGB1和ICAM-1的表达，起到保护肠黏膜和减少肠腔内细菌或内毒素的移位，从而减轻干热环境下中暑引起的全身性炎症反应。

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	董翔
	许琴
	高晓康
	李佳佳
	唐军
	康燕
	沈才福
	刘江伟

关键词 ：干热环境, 姜黄素, 中暑, 肠黏膜损伤, 炎症因子

基金资助:新疆维吾尔自治区自然科学基金面上项目（2015211C241）

通讯作者: 刘江伟（1970-），男，博士，主任医师，博士生导师，E-mail:ljw273273@163.com。

作者简介: 董翔（1982-），男，硕士研究生，实验师，E-mail: 49834420@qq.com。

引用本文:

董翔，许琴，高晓康，李佳佳，唐军，康燕，沈才福，刘江伟. 姜黄素预处理对干热环境下中暑大鼠肠黏膜相关蛋白及血清炎症因子的影响[J]. 中国急救医学, 2018, 38(10): 906-909.
Dong Xiang, Xu Qin, Gao Xiao-kang, Li Jia-jia, Tang Jun, Kang Yan, Shen Cai-fu, Liu Jiang-wei. Effect of curcumin pretreatment on heat stroke rat′s proteins related to intestinal mucosa and serum inflammatory factors in dry-heat environment. Chinese Journal of Critical Care Medicine, 2018, 38(10): 906-909.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2018.10.015 或 http://www.cnemergency.org.cn/CN/Y2018/V38/I10/906

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