大黄对脓毒症相关大鼠脑功能障碍的作用机制研究

doi:10.3969/j.issn.1002-1949.2017.10.002

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摘要目的：研究大黄对脓毒症相关大鼠脑功能障碍的作用及其可能作用机制。方法：将84只Wistar大鼠随机分为盲肠结扎对照（Sham）组（分A、B小组）、盲肠结扎穿孔术（CLP）组（分A、B小组）和大黄灌胃+CLP（R+CLP）组（分A、B小组）。R+CLP组给予大黄二甲基亚砜溶液经胃管灌胃，Sham组和CLP组仅给予二甲基亚砜溶液灌胃，通过CLP建立脓毒症大鼠模型。A组大鼠用于观察生存时间。B组大鼠分别于术后6、24、48h被处死，ELISA法测血清神经元特异性烯醇化酶（NSE）、S100β，右脑部分脑组织用于HE染色和免疫组化法测AQP-4表达情况，部分用ELISA法检测脑组织NF-κB、TNF-α、IL-6表达水平，左脑用于干湿质量法测含水量评价脑水肿情况。〖HTH〗结果〓〖HTSS〗①CLP组和R+CLP组脑组织含水量较Sham组明显增加；R+CLP组术后6h脑组织含水量较CLP组术后6 h显著降低。②CLP组和R+CLP组脑组织炎性因子（NF-κB、IL-6和TNF-α）水平较Sham组明显升高；R+CLP组术后24 h脑组织炎性因子水平较CLP组术后24 h明显降低。③CLP组和R+CLP组血清NSE、S100β含量较Sham组明显升高；R+CLP组术后24 h血清NSE、S100β含量较CLP组术后24 h明显降低。④光镜显示预使用大黄灌胃可减轻脓毒症后脑组织水肿程度，其中R+CLP组术后6 h脑组织水肿程度较CLP组术后6 h明显减轻。⑤脑组织免疫组化：预使用大黄灌胃可降低脑组织胶质细胞表达AQP-4水平，其中R+CLP组术后6 h脑组织水肿程度较CLP组术后6h明显降低。⑥R+CLP组不同时间点生存率较CLP组明显提高。结论：预使用大黄对大鼠脓毒症相关脑功能障碍有明显的保护作用。

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	郭阿茹
	马莉
	李杜鹏
	张蓓
	安宏

收稿日期: 2017-03-01

基金资助:北京协和医学基金会睿E（睿意）急诊医学研究专项基金（R2015003）

作者简介: 郭阿茹(1991-)，女，硕士研究生，E-mail:aruguo0126@163.com

引用本文:

郭阿茹，马莉，李杜鹏，张蓓，安宏. 大黄对脓毒症相关大鼠脑功能障碍的作用机制研究[J]. 中国急救医学, 2017, 37(10): 878-882.
Guo A-ru, Ma Li, Li Du-peng, Zhang Bei, An Hong. Effects of Rhubarb on brain dysfunction in septic rats. Chinese Journal of Critical Care Medicine, 2017, 37(10): 878-882.

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http://www.cnemergency.org.cn/CN/10.3969/j.issn.1002-1949.2017.10.002 或 http://www.cnemergency.org.cn/CN/Y2017/V37/I10/878

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